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A CONFOCAL MICROSCOPIC STUDY ON DENTINAL INFILTRATION OF ONE-BOTTLE ADHESIVE SYSTEMS AND SELF-ETCHING PRIMING SYSTEM BONDED TO CLASS V CAVITIES

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Abstract


Objective : The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM).

Material and Methods : Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group ¥°, cervical abrasion ; Group ¥±, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene^?) and Syntac^?Sprint^¢â(SS: VIVADENT)-, one self-etching priming system-CLEARFIL^^¢â SE BOND(SB; KURARAY)- and one multistep dentin bonding system-Scotchbond^¢â Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufactures instructions. Cavities were restored with Spectrum^?(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudially with a low-speed diamond disc. The resin dentin interfaces were microscopically observed using CLSM. The quality of resin-inflitrated dentin layers were evaluated by five dentists using 0-4 scale.

Results : Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins.
Statistical analysis using one-way ANOVA followed by Duncan¢¥s Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lowet than SBMP.

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